What question did this study set out to answer?

Purify and characterize a high-affinity soluble angiotensin-binding protein (sABP) from porcine liver cytosol to distinguish its properties and tissue distribution from plasma membrane receptors.

November 1, 1989European Journal of BiochemistryOpen Access

Purification and characterization of angiotensin‐binding protein from porcine liver cytosolic fraction

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Population

Porcine liver cytosolic fraction

Design

Preclinical

Authors

HHHiromi HagiwaraToin University of Yokohama NSNaoaki SugiuraLouisiana State University Health Sciences Center New Orleans KWKenichi WakitaYodogawa Christian Hospital

Discussion

Member takes

Implication

No immediate clinical implications from porcine model; leaves open sABP's role in human angiotensin signaling.

Key Points

Purify and characterize a high-affinity soluble angiotensin-binding protein (sABP) from porcine liver cytosol to distinguish its properties and tissue distribution from plasma membrane receptors.
Purified sABP from porcine liver cytosolic fraction using sequential ammonium sulfate fractionation, hydrophobic chromatography, ion-exchange chromatography, hydroxylapatite chromatography, and Mono Q ion-exchange chromatography.
Quantified specific binding activity using 125I-angiotensin II and estimated molecular mass by SDS-polyacrylamide gel electrophoresis and affinity labeling.
Assessed relative affinities across angiotensin analogues and examined sABP tissue distribution across peripheral organs and brain tissue.
Achieved a greater than 3,400-fold enrichment of specific angiotensin-binding activity, yielding a single 75-kDa band on SDS-PAGE consistent with affinity labeling estimates.
Observed identical sensitivity to reducing agents and a rank-order binding affinity ([Sar1, Ala8]angiotensin II > angiotensin III > angiotensin II > angiotensin I) similar to membrane-bound angiotensin II receptors.
Detected broad tissue distribution of sABP across both peripheral organs and the brain.

Structured PICO

Population

Porcine liver cytosolic fraction

Intervention

Purification and characterization of soluble angiotensin-binding protein (sABP)

Outcome

Identification, purification, and characterization of sABP

The study identifies and characterizes a novel soluble angiotensin-binding protein in porcine liver cytosol with similar binding properties to the angiotensin II receptor.

Cite This Study

Hagiwara et al. (1989) studied this question.

synapsesocial.com/papers/6a749f8e1078cf7de2b1cd75 https://doi.org/10.1111/j.1432-1033.1989.tb15129.x

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Purification and characterization of angiotensin‐binding protein from porcine liver cytosolic fraction

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Purification and characterization of angiotensin‐binding protein from porcine liver cytosolic fraction

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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