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February 11, 1991FEBS Letters70 citationsOpen Access

Identification of the protein and cDNA of the cardiac Na+ /H+ exchanger

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LFLarry FliegelCSClaude SardetJPJacques Pouysségur

Structured PICO

P
Population
Rabbit cardiac library and isolated sarcolemma
I
Intervention
Isolation of partial length cDNA clone and Western blot analysis with antibodies against a fusion protein
C
Comparator
Human Na+/H+ exchanger sequence
O
Outcome
Identification and characterization of the myocardial Na+/H+ exchanger protein and cDNAsurrogate

This basic science study successfully identified and characterized the rabbit cardiac Na+/H+ exchanger, showing high homology with the human form.

Abstract

We examined the myocardial form of the Na+/H+ exchanger. A partial length cDNA clone was isolated from a rabbit cardiac library and it encoded for a Na+/H+ exchange protein. In comparison with the human Na+/H+ exchanger, the sequence of the 5' end of the cDNA was highly conserved, much more than the 3' region, while the deduced amino acid sequence was also highly conserved. To further characterize the myocardial Na+/H+ exchange protein, we examined Western blots of isolated sarcolemma with antibody produced against a fusion protein of the Na+/H+ exchanger. The antibodies reacted with a sarcolemma protein of 50 kDa and with a protein of 70 kDa. The results show that the rabbit myocardium does possess a Na+/H+ exchanger protein homologous to the known human Na+/H+ exchanger.

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Cite This Study

Fliegel et al. (1991) studied this question.

synapsesocial.com/papers/6a74d8769c92392688b1e445https://doi.org/10.1016/0014-5793(91)80241-t
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Molecular Cloning and Functional Expression of the Cardiac Sarcolemmal Na <sup>+</sup> -Ca <sup>2+</sup> Exchanger1990 · 746 citations
  2. 2Molecular cloning and characterization of the human cardiac Na+/Ca2+ exchanger cDNA.1992 · 164 citations
  3. 3Expression of the Na-Ca exchanger in diverse tissues: a study using the cloned human cardiac Na-Ca exchanger1992 · 162 citations
  4. 4Distribution of the Na(+)-Ca2+ exchange protein in mammalian cardiac myocytes: an immunofluorescence and immunocolloidal gold-labeling study1992 · 200 citations
  5. 5Distribution of the Na+/Ca2+ exchange protein in developing rabbit myocytes1995 · 68 citations