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April 15, 1992The Journal of Cell Biology200 citationsOpen Access

Localization of Sodium-Calcium Exchangers in Mammalian Cardiac Sarcolemma and Transverse Tubules

Distribution of the Na(+)-Ca2+ exchange protein in mammalian cardiac myocytes: an immunofluorescence and immunocolloidal gold-labeling study

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Population

Mammalian cardiac myocytes (specifically guinea pig myocytes and canine cardiac Na(+)-Ca2+ exchanger)

Design

Preclinical

Authors

JFJ. S. FrankGMGiuliano A. MottinoDRDelyth M. Reid

Discussion

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Overview

The Na(+)-Ca2+ exchanger is abundantly located in the sarcolemma closest to the release of Ca2+, specifically in the transverse tubules.

Key Points

  • Determine the subcellular distribution and structural localization of the Na+-Ca2+ exchange protein within mammalian cardiac sarcolemma.
  • Employed immunofluorescence and immunoelectron microscopy with immunocolloidal gold labeling on guinea pig cardiac myocytes.
  • Utilized monoclonal and polyclonal antibodies recognizing extracellular-facing epitopes of the reconstituted cardiac Na+-Ca2+ exchanger.
  • Immunofluorescence revealed prominent labeling throughout the interconnected transverse tubular (T-tubule) network alongside patchy peripheral sarcolemmal labeling.
  • Immunoelectron microscopy confirmed localization to the sarcolemma with an apparent high density within the transverse tubules, positioning the calcium efflux system adjacent to intracellular calcium release sites.

Structured PICO

P
Population
Mammalian cardiac myocytes (specifically guinea pig myocytes and canine cardiac Na(+)-Ca2+ exchanger)
I
Intervention
Immunofluorescence and immunoelectron microscopy using polyclonal and monoclonal antibodies
O
Outcome
Location and distribution of the Na(+)-Ca2+ exchanger in cardiac sarcolemmasurrogate

The Na(+)-Ca2+ exchanger is abundantly located in the sarcolemma closest to the release of Ca2+, specifically in the transverse tubules.

Cite This Study

Frank et al. (1992) studied this question.

synapsesocial.com/papers/6a70d46745e51b8c20273823https://doi.org/10.1083/jcb.117.2.337
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