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July 1, 1979Proceedings of the National Academy of SciencesOpen Access

A normal cell protein similar in structure and function to the avian sarcoma virus transforming gene product.

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Authors

MCMarc S. CollettUniversity of Nebraska–LincolnEEE EriksonNorthwestern UniversityAPA F PurchioUniversity of Toledo Medical Center

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Overview

Biochemical analysis reveals a normal cellular phosphoprotein related to viral pp60src in avian and mammalian cells, indicating shared mechanisms in growth.

Key Points

  • To identify and biochemically characterize a normal cellular protein counterpart related to the avian sarcoma virus transforming gene product pp60src.
  • Immunoprecipitated radiolabeled cell extracts from normal avian and mammalian cells using antisera from mice and rabbits bearing avian sarcoma virus-induced tumors.
  • Analyzed the molecular weight, phosphorylation site distribution, and associated protein kinase enzymatic activity of the isolated cellular protein.
  • Identified a normal cellular 60,000-dalton phosphoprotein (pp60sarc) across avian and mammalian cells displaying structural similarity to viral pp60src.
  • Resolved two conserved phosphorylation regions: a phosphoserine residue on the NH2-terminal 60% and a phosphothreonine residue on the COOH-terminal 40% of the polypeptide.
  • Demonstrated an associated protein kinase activity in normal pp60sarc analogous to the enzymatic function of the viral transforming protein.

Cite This Study

Collett et al. (1979) studied this question.

synapsesocial.com/papers/6a74fef4d93df8ecca092f7fhttps://doi.org/10.1073/pnas.76.7.3159
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Uninfected vertebrate cells contain a protein that is closely related to the product of the avian sarcoma virus transforming gene (src).1979 · 385 citations
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