Angiopoietin-1 and -2 and vascular endothelial growth factor expression in ovarian grafts after cryopreservation using two methods

Figures

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  Figure 1. Flow diagram of experimental design. After 1 week of cryopreservation (vitrification [Vit] or slow freezing [SF]), a mouse ovary was analyzed for angiopoietin-1, -2, and VEGF; the other ovary was autotransplanted beneath the abdominal wall. After a 2-week autotransplantation period, angiopoietin-1, -2, and VEGF were assayed using RT-PCR. Cont, control; RT-PCR, reverse transcriptase-polymerase chain reaction; VEGF, vascular endothelial growth factor.

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  Figure 2. Autotransplanted mouse ovary beneath the abdominal wall (arrow) to be assayed after 2 weeks. The ovary was attached to the peritoneum by fine fibrous tissue.

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  Figure 3. Reverse transcriptase-polymerase chain reaction analysis of vascular endothelial growth factor-A (VEGF-A; A), angiopoietin-1 (Angpt-1; B), and angiopoietin-2 (Angpt-2; C) mRNA levels after 1 week of vitrification or slow-freezing compared to the control group and quantitative analysis of each group. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as the internal standard. Values are presented as mean±standard error of three independent experiments. EFS, ethylene-glycol, ficoll, and sucrose solution. a)p<0.05 for the control vs. cryopreservation groups (EFS and slow-freezing).

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  Figure 4. Reverse transcriptase-polymerase chain reaction analysis of post-transplant (PT) vascular endothelial growth factor-A (VEGF-A; A), angiopoietin-1 (Angpt-1; B), and angiopoietin-2 (Angpt-2; C) mRNA levels after 2 weeks of autotransplantation beneath the abdominal wall. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as the internal standard. Values are presented as mean±standard error of three independent experiments. EFS, ethylene-glycol, ficoll, and sucrose solution. a)p<0.05 for the control vs. cryopreservation groups (EFS and slow-freezing).