Effects of Angiopoietin-2 on Transplanted Mouse Ovarian Tissue

Figures

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  Fig 1. Histology of ovarian grafts (original magnification ×100). The horizontal axis represents the days after OT transplantation. The vertical axis represents the control and Ang2-treated groups (control, no treatment of Ang2; 50 ng Ang2, injection of 50 ng/kg Ang2; 500 ng Ang2, injection of 500 ng/kg Ang2; D2, D7, D21 and D42, 2, 7, 21 and 42 days after transplantation, respectively).

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  Fig 2. The results of the comparison of (a) the mean numbers of follicles, (b) intact G1 follicle ratios, (c) serum FSH levels, and (d) apoptotic follicle ratios in the OT grafts after Ang2 treatment according to post-transplantation durations. The statistical difference was considered significant when the p value was <0.05 (control, no treatment of Ang2; 50 ng Ang2, injection of 50 ng/kg Ang2; 500 ng Ang2, injection of 500 ng/kg Ang2). Different superscript letters indicate statistically significant differences (p < 0.05), and the superscripts were used for each group of the same transplantation duration separately.

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  Fig 3. Representative appearance of good and poor vascularization and angiogenesis of OT grafts transplanted into the subcutaneous pockets and recovered on D7 after grafting. (a, b) Good formation of new blood vessels. Several new vessels branching to the OT graft are clearly visible and have a healthy appearance. Three follicles were yellow-white in color in the red OT grafts. (c, d) Poor vascularization. Rare vessels were visible and the OT graft seems to be necrotized and/or degenerated without visible follicles.

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  Fig 4. Representative photographs of CD31(+) vessels (dark-brown color) in OT grafts (original magnification ×400). The horizontal axis represents the days after OT transplantation. The vertical axis represents the control and Ang2-treated groups (control, no treatment of Ang2; 50 ng Ang2, injection of 50 ng/kg Ang2; 500 ng Ang2, injection of 500 ng/kg Ang2; D2, D7, D21 and D42, 2, 7, 21 and 42 days after transplantation, respectively).

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  Fig 5. Comparison of the mean number of CD31(+) vessels in the OT grafts after Ang2 treatment according to post-transplantation durations. The statistical difference was considered significant when the p value was <0.05 (control, no treatment of Ang2; 50 ng Ang2, injection of 50 ng/kg Ang2; 500 ng Ang2, injection of 500 ng/kg Ang2). Different superscript letters indicate statistically significant differences (p < 0.05), and the superscripts were used for each group of the same transplantation duration separately.