What question did this study set out to answer?

To identify the ultrastructural dynamics and exocytic mechanisms responsible for resealing mechanically induced plasma membrane disruptions.

December 15, 1995The Journal of Cell BiologyOpen Access

Vesicle accumulation and exocytosis at sites of plasma membrane disruption.

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Authors

KMKatsuya MiyakeInternational University of Health and Welfare PMPaul L. McNeilAugusta University

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Implication

In vitro study demonstrates rapid vesicle accumulation and localized exocytosis in mechanically wounded cells, indicating that targeted membrane fusion drives cell wound resealing.

Key Points

To identify the ultrastructural dynamics and exocytic mechanisms responsible for resealing mechanically induced plasma membrane disruptions.
Marked mechanical disruption sites using horseradish peroxidase (HRP) and evaluated structural responses using transmission and scanning electron microscopy.
Quantified calcium-dependent exocytosis and spatial membrane remodeling using FM1-43 dye fluorescence assays and confocal microscopy.
Assessed post-injury alterations in cell dimensions using flow cytometry forward-scatter measurements.
Vesicles of diverse sizes accumulated within seconds around the disruption site, accompanied by the rapid appearance of surface microvilli-like projections overlying the wound.
FM1-43 assays and confocal microscopy revealed localized, calcium-dependent exocytosis originating from endosomal/lysosomal compartments directly at the cortical wound site rather than globally.
Flow cytometry detected an injury-induced increase in forward scatter, confirming that local membrane fusion increases overall cell surface area during repair.

Cite This Study

Miyake et al. (1995) studied this question.

synapsesocial.com/papers/6a754aae96d04a2f43f5899c https://doi.org/10.1083/jcb.131.6.1737

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Implication

In vitro study demonstrates rapid vesicle accumulation and localized exocytosis in mechanically wounded cells, indicating that targeted membrane fusion drives cell wound resealing.

Vesicle accumulation and exocytosis at sites of plasma membrane disruption.

Discussion

Member takes

Implication

Key Points

Cite This Study

Vesicle accumulation and exocytosis at sites of plasma membrane disruption.

Discussion

Member takes

Implication

Key Points

Cite This Study

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