What question did this study set out to answer?

To determine the molecular and cellular mechanisms governing calcium-dependent plasma membrane resealing after mechanical disruption.

January 21, 1994Science

Cell Membrane Resealing by a Vesicular Mechanism Similar to Neurotransmitter Release

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Authors

RSRichard A. SteinhardtUniversity of California, Berkeley GBGuo‐Qiang BiUniversity of Science and Technology of China JAJanet M. AldertonUniversity of California, Berkeley

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Implication

Experimental study demonstrates calcium-dependent membrane resealing in fibroblasts and embryos, indicating a vesicular mechanism analogous to neurotransmitter release.

Key Points

To determine the molecular and cellular mechanisms governing calcium-dependent plasma membrane resealing after mechanical disruption.
Measured dye loss kinetics following micropuncture injury in sea urchin embryos and 3T3 fibroblasts under varying calcium and magnesium conditions.
Assessed membrane resealing competency following pharmacological inhibition of multifunctional calcium/calmodulin kinase and kinesin-mediated vesicle transport.
Investigated the functional involvement of synaptobrevin and SNAP-25 by microinjecting botulinum neurotoxins B and A prior to injury.
Rapid resealing of punctured membranes required extracellular calcium and was competitively antagonized by magnesium in both embryonic and mammalian cells.
Inhibition of kinesin motor proteins or multifunctional calcium/calmodulin kinase significantly disrupted vesicle availability and prevented membrane resealing.
Targeted proteolysis of synaptobrevin and SNAP-25 by botulinum neurotoxins A and B blocked repair, indicating that vesicle docking and SNARE-mediated fusion drive membrane resealing.

Cite This Study

Steinhardt et al. (1994) studied this question.

synapsesocial.com/papers/6a754aae96d04a2f43f589a2 https://doi.org/10.1126/science.7904084

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Implication

Experimental study demonstrates calcium-dependent membrane resealing in fibroblasts and embryos, indicating a vesicular mechanism analogous to neurotransmitter release.

Cell Membrane Resealing by a Vesicular Mechanism Similar to Neurotransmitter Release

Discussion

Member takes

Implication

Key Points

Cite This Study

Cell Membrane Resealing by a Vesicular Mechanism Similar to Neurotransmitter Release

Discussion

Member takes

Implication

Key Points

Cite This Study

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