December 7, 2007Journal of Biological ChemistryOpen Access

Structural Kinetics of Cardiac Troponin C Mutants Linked to Familial Hypertrophic and Dilated Cardiomyopathy in Troponin Complexes

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Structured PICO

Population

Reconstituted troponin complexes containing human cardiac troponin C (cTnC) double or triple mutants with tryptophanless cTnI and cTnT

Intervention

Triple mutants containing L12W and N51C labeled with 1,5-IAEDANS, and either L29Q or G159D mutations

Comparator

Double mutant cTnC(L12W/N51C) labeled with 1,5-IAEDANS at Cys-51 (control)

Outcome

Kinetics of structural transitions (opening and closing rates) in the regulatory N-domain of cTnC triggered by Ca(2+) binding and dissociationsurrogate

Cardiomyopathy-linked mutations L29Q and G159D in cardiac troponin C alter the kinetics of Ca(2+)-triggered structural transitions and antagonize PKA phosphorylation signaling.

Discussion

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Implication

These troponin C mutations may disrupt contractile regulation in cardiomyopathy; hypothesis-generating and should not yet change practice.

Cite This Study

Dong et al. (2007) studied this question.

synapsesocial.com/papers/6a757e26cbb3fa1e5bdbe262 https://doi.org/10.1074/jbc.m703822200

View Full Paper

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Implication

These troponin C mutations may disrupt contractile regulation in cardiomyopathy; hypothesis-generating and should not yet change practice.

Structural Kinetics of Cardiac Troponin C Mutants Linked to Familial Hypertrophic and Dilated Cardiomyopathy in Troponin Complexes

Structured PICO

Discussion

Implication

Cite This Study

Structural Kinetics of Cardiac Troponin C Mutants Linked to Familial Hypertrophic and Dilated Cardiomyopathy in Troponin Complexes

Structured PICO

Discussion

Implication

Cite This Study

Also Consider

Also Consider