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August 7, 2026ACS Biomaterials Science & Engineering

Engineered Deoxyribonucleic Acid-Tunable Spheroid Platform for Analysis of Tumor-Macrophage Interactions

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Authors

SSSven A. SaemundssonTATaylor R. AusecBBBryce M. Bower

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Overview

Randomized trial investigates tumor-macrophage interactions in engineered spheroids, suggesting new insights into immune modulation.

Key Points

  • The aim is to investigate how engineered tumor spheroids influence macrophage behavior under varying conditions of spacing and hypoxia.
  • Utilized a DNA-programmable spheroid platform to create 4T1 tumor spheroids with adjustable intercellular spacing and hypoxia gradients.
  • Compared infiltration and polarization of immortalized RAW 264.7 macrophages and primary bone marrow-derived macrophages (BMDMs).
  • Analyzed soluble factors' role in macrophage activation in both 2D and 3D environments.
  • BMDMs infiltrated spheroids more rapidly than RAW macrophages, reaching maximal infiltration at 24 hours.
  • RAW macrophages showed hypoxia-dependent polarization at 24 hours, with lower hypoxia levels leading to a higher CD80/CD206 ratio.
  • BMDMs had a muted response to hypoxia, with inflammation peaking at 24 hours and subsiding thereafter.

Cite This Study

Saemundsson et al. (2026) studied this question.

synapsesocial.com/papers/6a758b93847ab6d26c01ef91https://doi.org/10.1021/acsbiomaterials.6c00195
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Also Consider

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  5. 5Modeling 3D Tumor Invasiveness to Modulate Macrophage Phenotype in a Human‐Based Hydrogel Platform2024 · 1 citations