What question did this study set out to answer?

To determine how transcriptional coactivators p300 and CBP regulate the activity, post-translational modification, and stability of SREBP family transcription factors.

March 15, 2003Molecular and Cellular Biology

Coactivator-Dependent Acetylation Stabilizes Members of the SREBP Family of Transcription Factors

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Authors

VGValeria GiandomenicoUppsala University MSMaria SimonssonLinköping University EGEva GrünroosLudwig Cancer Research

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Implication

Biochemical analysis reveals that p300-mediated acetylation stabilizes SREBP transcription factors by blocking ubiquitination, indicating a novel coactivator mechanism for lipid gene regulation.

Key Points

To determine how transcriptional coactivators p300 and CBP regulate the activity, post-translational modification, and stability of SREBP family transcription factors.
Assayed acetylation of SREBP1a and SREBP2 mediated by the intrinsic acetyltransferase activity of coactivators p300 and CBP.
Evaluated SREBP protein expression, stability, and ubiquitination following p300 coexpression and targeted mutagenesis of the acetylated lysine residue within the SREBP1a DNA-binding domain.
p300 and CBP acetylate SREBPs, with the modification occurring at a specific lysine residue within the DNA-binding domain of SREBP1a.
Coexpression with p300 substantially increases SREBP1a and SREBP2 protein levels via an acetyltransferase-dependent mechanism.
Acetylation or mutation of the specific lysine residue in SREBP1a prevents ubiquitination at that same site, thereby stabilizing the protein.

Cite This Study

Giandomenico et al. (2003) studied this question.

synapsesocial.com/papers/6a75900d1cd7cac2185ffd5e https://doi.org/10.1128/mcb.23.7.2587-2599.2003

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Implication

Biochemical analysis reveals that p300-mediated acetylation stabilizes SREBP transcription factors by blocking ubiquitination, indicating a novel coactivator mechanism for lipid gene regulation.

Coactivator-Dependent Acetylation Stabilizes Members of the SREBP Family of Transcription Factors

Discussion

Member takes

Implication

Key Points

Cite This Study

Coactivator-Dependent Acetylation Stabilizes Members of the SREBP Family of Transcription Factors

Discussion

Member takes

Implication

Key Points

Cite This Study

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