What question did this study set out to answer?

To identify the specific conserved structural domains of Ets1 required for cellular nuclear localization and in vitro DNA-binding activity.

December 1, 1989Molecular and Cellular BiologyOpen Access

Definition of an Ets1 Protein Domain Required for Nuclear Localization in Cells and DNA-Binding Activity In Vitro

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Authors

KBKim E. BoulukosUniversité Côte d'Azur PPPhilippe PognonecUniversité Côte d'Azur BRBertrand RabaultCentre National de la Recherche Scientifique

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Implication

Experimental deletion analysis reveals that the carboxy-terminal domain of Ets1 directs nuclear localization and DNA binding, indicating a dual-function regulatory role across Ets proteins.

Key Points

To identify the specific conserved structural domains of Ets1 required for cellular nuclear localization and in vitro DNA-binding activity.
Performed targeted deletion mutagenesis across two conserved identity domains shared between Ets1 and Ets2.
Assessed mutated Ets1 variants for intracellular nuclear transport in cells and sequence-specific DNA binding in vitro.
Integrity of the carboxy-terminal domain, shared with Ets2, Elk, and Erg proteins, was strictly required for both nuclear targeting and in vitro DNA-binding capability.
Deletion of this conserved carboxy-terminal region abolished the protein's ability to localize to the cell nucleus and interact with DNA targets.

Cite This Study

Boulukos et al. (1989) studied this question.

synapsesocial.com/papers/6a75b1aff1e99ff85ab20944 https://doi.org/10.1128/mcb.9.12.5718-5721.1989

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Implication

Experimental deletion analysis reveals that the carboxy-terminal domain of Ets1 directs nuclear localization and DNA binding, indicating a dual-function regulatory role across Ets proteins.

Definition of an Ets1 Protein Domain Required for Nuclear Localization in Cells and DNA-Binding Activity In Vitro

Discussion

Member takes

Implication

Key Points

Cite This Study

Definition of an Ets1 Protein Domain Required for Nuclear Localization in Cells and DNA-Binding Activity In Vitro

Discussion

Member takes

Implication

Key Points

Cite This Study

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