What question did this study set out to answer?

To determine whether tissue injury in very severe COPD stimulates alveolar elastin gene expression as an active repair response.

April 8, 2009European Respiratory JournalOpen Access

Elastin expression in very severe human COPD

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Authors

GDGaëtan DesléeUniversité de Reims Champagne-Ardenne JWJason C. WoodsCincinnati Children's Hospital Medical Center CMCamille M. MooreGladstone Institutes

Discussion

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Implication

Comparative tissue analysis reveals elevated alveolar elastin expression in very severe COPD, indicating an active but potentially ineffective tissue repair response.

Key Points

To determine whether tissue injury in very severe COPD stimulates alveolar elastin gene expression as an active repair response.
Analyzed lung tissue from 43 subjects: 11 with stage 4 COPD, 10 with stage 2–3 COPD, 10 organ donors, and 12 non-COPD controls.
Measured elastin mRNA expression using real-time RT-PCR and localized transcripts via in situ hybridization.
Quantified alveolar elastic fibers using Hart's staining on frozen inflated lung sections from 11 stage 4 COPD patients and 3 donors.
Elastin mRNA expression was significantly increased by 12-fold in stage 4 COPD lungs compared to donors, non-COPD controls, and stage 2–3 COPD, localizing to alveolar walls.
Alveolar elastic fibers occupied a significantly greater volume fraction of lung tissue in stage 4 COPD compared to donors (p<0.01).
Total elastic fiber content per overall lung volume and total desmosine content were not increased in stage 4 COPD lungs.

Cite This Study

Deslée et al. (2009) studied this question.

synapsesocial.com/papers/6a760a82d5b06e2c1e613c78 https://doi.org/10.1183/09031936.00123008

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April 8, 2009European Respiratory JournalOpen Access

Elastin expression in very severe human COPD

View Full Paper

Ask AI

Bookmark

Authors

GDGaëtan DesléeUniversité de Reims Champagne-Ardenne JWJason C. WoodsCincinnati Children's Hospital Medical Center CMCamille M. MooreGladstone Institutes

Discussion

Member takes

Implication

Comparative tissue analysis reveals elevated alveolar elastin expression in very severe COPD, indicating an active but potentially ineffective tissue repair response.

Key Points

To determine whether tissue injury in very severe COPD stimulates alveolar elastin gene expression as an active repair response.
Analyzed lung tissue from 43 subjects: 11 with stage 4 COPD, 10 with stage 2–3 COPD, 10 organ donors, and 12 non-COPD controls.
Measured elastin mRNA expression using real-time RT-PCR and localized transcripts via in situ hybridization.
Quantified alveolar elastic fibers using Hart's staining on frozen inflated lung sections from 11 stage 4 COPD patients and 3 donors.
Elastin mRNA expression was significantly increased by 12-fold in stage 4 COPD lungs compared to donors, non-COPD controls, and stage 2–3 COPD, localizing to alveolar walls.
Alveolar elastic fibers occupied a significantly greater volume fraction of lung tissue in stage 4 COPD compared to donors (p<0.01).
Total elastic fiber content per overall lung volume and total desmosine content were not increased in stage 4 COPD lungs.

Cite This Study

Deslée et al. (2009) studied this question.

synapsesocial.com/papers/6a760a82d5b06e2c1e613c78 https://doi.org/10.1183/09031936.00123008

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