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February 25, 2005The Journal of PhysiologyOpen Access

Calcium transients in developing mouse skeletal muscle fibres

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Population

Intact skeletal muscle fibres enzymatically dissociated from mice of different ages (7, 10, 15 and 42 days)

Comparison

Measurement of Ca transients elicited by action… vs Comparison between different age groups

Design

Preclinical

Authors

JCJoana CapoteUniversity of California, Los AngelesPBPura BolañosInstituto Venezolano de Investigaciones CientíficasRSR.P. SchuhmeierUniversität Ulm

Discussion

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Implication

Hypothesis-generating for developmental Ca2+ handling in mice; leaves open translation to human cardiac or skeletal muscle.

Structured PICO

P
Population
Intact skeletal muscle fibres enzymatically dissociated from mice of different ages (7, 10, 15 and 42 days)
I
Intervention
Measurement of Ca(2+) transients elicited by action potentials using MagFluo-4, exposure to Ca(2+)-free solutions, and cyclopiazonic acid (1 µM)
C
Comparator
Comparison between different age groups (7, 10, 15 days vs 42 days/adult)
O
Outcome
Rise time and decay time constants of Ca(2+) transientssurrogate

Developing mouse skeletal muscle fibres exhibit slower Ca(2+) transient kinetics compared to adult fibres, suggesting a larger relative contribution of the SR Ca(2+) pump and lower expression of myoplasmic Ca(2+) buffers in younger animals.

Cite This Study

Capote et al. (2005) studied this question.

synapsesocial.com/papers/6a761452cd662f67b2fbd86fhttps://doi.org/10.1113/jphysiol.2004.081034
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Also Consider

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  1. 1Voltage-dependent Ca2+ Fluxes in Skeletal Myotubes Determined Using a Removal Model Analysis2003 · 42 citations
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  3. 3Purification and Characterization of Two Ryanodine-Binding Protein Isoforms from Sarcoplasmic Reticulum of Bullfrog Skeletal Muscle11992 · 81 citations
  4. 4The amplitude and time course of the myoplasmic free [Ca2+] transient in fast-twitch fibers of mouse muscle.1996 · 89 citations
  5. 5Calcium transients in intact rat skeletal muscle fibers in agarose gel1995 · 41 citations