July 1, 1999The Journal of General PhysiologyOpen Access

Calcium Release Flux Underlying Ca2+ Sparks of Frog Skeletal Muscle

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Population

Single frog skeletal muscle fibers

Comparison

Modified algorithm for the calculation of Ca2+… vs Simulated fluorescence due to release of Ca2+…

Design

Preclinical

Authors

EREduardo Rı́osElectrophysiology MSMichael D. SternElectrophysiology AGAdom GonzálezInstituto Venezolano de Investigaciones Científicas

Discussion

Member takes

Implication

Enables Ca2+ flux quantification in skeletal muscle sparks; extends analytical methods but leaves open cardiac translation.

Structured PICO

Population

Single frog skeletal muscle fibers (voltage clamped in a two-Vaseline gap chamber or permeabilized and immersed in fluo-3-containing internal solution)

Intervention

Modified algorithm for the calculation of Ca2+ release flux underlying Ca2+ sparks

Comparator

Simulated fluorescence due to release of Ca2+ from a spherical source

Outcome

Ca2+ release current, source diameter, and open timesurrogate

A modified algorithm successfully estimated Ca2+ release flux underlying Ca2+ sparks in frog skeletal muscle, suggesting a multichannel origin of sparks.

Limitations

Fewer sparks were obtained in permeabilized fibers, degrading algorithm performance
Real sparks differ from simulated ones mainly in having greater width, which could reflect limitations in spatial resolution

Cite This Study

Rı́os et al. (1999) studied this question.

synapsesocial.com/papers/6a761454cd662f67b2fbd877 https://doi.org/10.1085/jgp.114.1.31

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Calcium Release Flux Underlying Ca2+ Sparks of Frog Skeletal Muscle

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

Calcium Release Flux Underlying Ca2+ Sparks of Frog Skeletal Muscle

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

Also Consider

Also Consider