Key result
Depolarization-induced calcium release in rat skeletal muscle fibres lacked discrete calcium sparks and showed a voltage-independent peak-to-steady gradient ratio of 2.3, unlike frog fibres.
Population
Single muscle fibres mechanically dissected from fast twitch muscle of rats or frogs, and myotubes from…
Comparison
Low voltage depolarizations and application of… vs Comparison between frog, adult rat, and…
Design
Preclinical
Authors
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Minimal CICR in rat skeletal muscle E-C coupling; challenges frog models and leaves open human translation.
The study suggests that in adult rat skeletal muscle fibers, there is little contribution by calcium-induced calcium release to Ca2+ release triggered by depolarization, unlike in frog muscle.
Shirokova et al. (1998) studied this question. Low voltage depolarizations and caffeine application vs. Frog skeletal muscle fibres was evaluated on Ca2+ sparks and depolarization-induced gradients of fluorescence. Depolarization-induced calcium release in rat skeletal muscle fibres lacked discrete calcium sparks and showed a voltage-independent peak-to-steady gradient ratio of 2.3, unlike frog fibres.
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