What question did this study set out to answer?

To determine whether chronic activation of AMPK alters uncoupling protein-3 expression, metabolic enzyme profiles, and myosin heavy chain fibre types in fast-twitch skeletal muscle.

August 1, 2003The Journal of Physiology

AMPK activation increases uncoupling protein-3 expression and mitochondrial enzyme activities in rat muscle without fibre type transitions

View Full Paper

Ask AI

Bookmark

Authors

CPCharles T. PutmanUniversity of Alberta MKMónika KiricsiUniversity of Szeged JPJ. PearceyUniversity of Alberta

Discussion

Member takes

Overview

Animal study demonstrates that chronic AMPK activation enhances mitochondrial enzymes and UCP-3 in rat muscle, suggesting metabolic and structural phenotypes adapt independently.

Key Points

To determine whether chronic activation of AMPK alters uncoupling protein-3 expression, metabolic enzyme profiles, and myosin heavy chain fibre types in fast-twitch skeletal muscle.
Sprague-Dawley rats received daily injections of the AMPK activator AICAR or a vehicle control for 28 days.
Fast-twitch tibialis anterior muscles were analyzed for UCP-3 mRNA and protein, metabolic enzyme activities, and MHC fibre phenotype via immunohistochemistry and electrophoresis.
AICAR treatment elevated UCP-3 mRNA by 1.6 ± 0.1-fold (P < 0.006) and UCP-3 protein by 3.3 ± 0.2-fold (P < 0.0001).
Mitochondrial enzyme activities of citrate synthase and 3-hydroxyacyl-CoA-dehydrogenase increased 1.6-fold (P < 0.006), whereas lactate dehydrogenase activity fell to 80% of control (P < 0.02).
No changes were observed in glycolytic enzyme activities (glyceraldehydephosphate dehydrogenase or phosphofructokinase) or MHC-based fibre type composition.

Cite This Study

Putman et al. (2003) studied this question.

synapsesocial.com/papers/6a761edd9aebbbed366143cb https://doi.org/10.1111/j.1469-7793.2003.00169.x

View Full Paper

Ask AI

Bookmark

August 1, 2003The Journal of Physiology

AMPK activation increases uncoupling protein-3 expression and mitochondrial enzyme activities in rat muscle without fibre type transitions

View Full Paper

Ask AI

Bookmark

Authors

CPCharles T. PutmanUniversity of Alberta MKMónika KiricsiUniversity of Szeged JPJ. PearceyUniversity of Alberta

Discussion

Member takes

Overview

Animal study demonstrates that chronic AMPK activation enhances mitochondrial enzymes and UCP-3 in rat muscle, suggesting metabolic and structural phenotypes adapt independently.

Key Points

To determine whether chronic activation of AMPK alters uncoupling protein-3 expression, metabolic enzyme profiles, and myosin heavy chain fibre types in fast-twitch skeletal muscle.
Sprague-Dawley rats received daily injections of the AMPK activator AICAR or a vehicle control for 28 days.
Fast-twitch tibialis anterior muscles were analyzed for UCP-3 mRNA and protein, metabolic enzyme activities, and MHC fibre phenotype via immunohistochemistry and electrophoresis.
AICAR treatment elevated UCP-3 mRNA by 1.6 ± 0.1-fold (P < 0.006) and UCP-3 protein by 3.3 ± 0.2-fold (P < 0.0001).
Mitochondrial enzyme activities of citrate synthase and 3-hydroxyacyl-CoA-dehydrogenase increased 1.6-fold (P < 0.006), whereas lactate dehydrogenase activity fell to 80% of control (P < 0.02).
No changes were observed in glycolytic enzyme activities (glyceraldehydephosphate dehydrogenase or phosphofructokinase) or MHC-based fibre type composition.

Cite This Study

Putman et al. (2003) studied this question.

synapsesocial.com/papers/6a761edd9aebbbed366143cb https://doi.org/10.1111/j.1469-7793.2003.00169.x

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

View Full Paper

Ask AI

Bookmark