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March 1, 1963Experimental Biology and Medicine

Antibodies to Rabbit  -Globulin After Immunizing with Various Preparations of Autologous  -Globulin.

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Authors

RWRalph C. WilliamsBoston UniversityHKH. G. KunkelRockefeller University

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Implication

Animal study demonstrates that immunization with autologous gamma-globulin induces rheumatoid factor-like antibodies in rabbits, highlighting pathways underlying autoimmune antibody formation.

Key Points

  • Investigate the production, specificity, and immunological characteristics of anti-gamma-globulin antibodies induced in rabbits using various autologous gamma-globulin preparations and antigen complexes.
  • Immunized rabbits with horse spleen ferritin in Freund's adjuvant, autologous antibody-ferritin complexes, native autologous gamma-globulin, or papain-split autologous gamma-globulin.
  • Assayed antibody responses and cross-reactivity using sensitized red cell agglutination, sensitized sheep cell tests, agar gel precipitation, and direct and indirect Coombs' tests.
  • Immunization with autologous papain-split gamma-globulin produced considerably higher anti-gamma-globulin titers compared to native autologous gamma-globulin or ferritin immunization alone.
  • Induced antibodies exhibited both 7S and 19S sedimentation characteristics, displayed individual gamma-globulin specificity, and exhibited strong cross-reactivity with human gamma-globulin.
  • Treated animals demonstrated positive direct and indirect Coombs' reactions, closely mirroring the serological features of human rheumatoid factors.

Cite This Study

Williams et al. (1963) studied this question.

synapsesocial.com/papers/6a766018c5ab23c76cebfee4https://doi.org/10.3181/00379727-112-28104
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Species Specificity of Globulins in Reactions with Rheumatoid Arthritis Serum1963 · 14 citations
  2. 2CARDIAC AUTOANTIBODIES1968 · 14 citations
  3. 3THE LOCALIZATION OF ANTI‐HEART ANTIBODIES TO HEART TISSUE IN RATS AND RABBITS WITH SPECIAL REFERENCE TO THE INDUCTION OF GRANULOMA OF ASCHOFF BODY TYPE1970 · 5 citations
  4. 4A Comprehensive and Quantitative Analysis of the Major Specificities in Rabbit Antithymocyte Globulin Preparations2013 · 115 citations
  5. 5Identification of γE-Antibodies as a Carrier of Reaginic Activity1967 · 457 citations