What question did this study set out to answer?

This research investigates how calcium ion availability affects the force and velocity in individual cardiac muscle cells.

February 1, 1977The Journal of General PhysiologyOpen Access

Force velocity relations of single cardiac muscle cells: calcium dependency.

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Population

Single cardiac muscle cells isolated by microdissection, with spontaneous activity suppressed by EGTA…

Comparison

Iontophoretically released calcium ions vs Varying amounts of calcium ions and different…

Design

Preclinical

Authors

NCNora M. De ClerckUniversity of Antwerp VCVictor ClaesUniversity of Antwerp DBD L BrutsaertBrigham and Women's Hospital

Discussion

Member takes

Implication

Supports calcium dependence of isolated cardiomyocyte mechanics across ionic strengths; leaves open translation to intact hearts or clinical practice.

Key Points

This research investigates how calcium ion availability affects the force and velocity in individual cardiac muscle cells.
Isolated cardiac preparations using microdissection and EGTA buffering to suppress spontaneous activity.
Calcium ions were released iontophoretically, and contractions were measured under varying conditions.
Simultaneous measurements of force, muscle length, and shortening velocity were conducted during isometric and isotonic contractions.
Peak force and shortening velocity depend on calcium ion concentration in both low and high ionic strength mediums.
Force-velocity relationships show time independence during significant phases of shortening.
No barrier effect on calcium diffusion was detected, confirming consistent mechanical performance regardless of micropipette placement.

Structured PICO

Population

Single cardiac muscle cells isolated by microdissection, with spontaneous activity suppressed by EGTA buffering

Intervention

Iontophoretically released calcium ions

Comparator

Varying amounts of calcium ions and different sites of release (single vs. two micropipettes)

Outcome

Mechanical performance (force development, muscle length, and shortening velocity)surrogate

In single cardiac muscle cells, mechanical performance including peak force and shortening velocity is directly dependent on the amount of calcium ions.

Cite This Study

Clerck et al. (1977) studied this question.

synapsesocial.com/papers/6a766eec3da069e66cbd3100 https://doi.org/10.1085/jgp.69.2.221

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Implication

Supports calcium dependence of isolated cardiomyocyte mechanics across ionic strengths; leaves open translation to intact hearts or clinical practice.

Force velocity relations of single cardiac muscle cells: calcium dependency.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Force velocity relations of single cardiac muscle cells: calcium dependency.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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