Translation in Saccharomyces cerevisiae: initiation factor 4E-dependent cell-free system.
Population
Saccharomyces cerevisiae cells
Comparison
Mutagenized eIF-4E gene introduced on a plasmid vs Wild-type eIF-4E gene
Design
Preclinical
Authors
Discussion
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Member takes
Implication
Provides a yeast model for eIF-4E-dependent translation; leaves open relevance to mammalian cardiovascular protein synthesis.
Structured PICO
P
Population
Saccharomyces cerevisiae cells
I
Intervention
Mutagenized eIF-4E gene introduced on a plasmid
C
Comparator
Wild-type eIF-4E gene
O
Outcome
Phenotype and temperature sensitivitysurrogate
The study developed a temperature-sensitive yeast strain (4-2) that demonstrates the dependence of cell-free translation systems on exogenous eIF-4E for certain mRNAs.
Cite This Study
Altmann et al. (1989) studied this question.