What question did this study set out to answer?

To determine whether downstream signaling components beyond cAMP formation, specifically cAMP-dependent protein kinase and its substrate phospholamban, are altered in failing human myocardium.

November 1, 1994Cardiovascular Research

cAMP concentrations, cAMP dependent protein kinase activity, and phospholamban in non-failing and failing myocardium

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Population

Failing human hearts (ischaemic and dilated cardiomyopathy) and non-failing donor hearts

Design

Other

Authors

MBMichael BöhmUniversity of Cologne BRB. ReigerUniversity of Cologne RSRobert H. G. SchwingerHeart Failure & Transplant

Discussion

Member takes

Implication

Reduced cAMP predominates in HF without PKA or phospholamban changes; hypothesis-generating for upstream targeting, no practice implications.

Key Points

To determine whether downstream signaling components beyond cAMP formation, specifically cAMP-dependent protein kinase and its substrate phospholamban, are altered in failing human myocardium.
Measured cAMP concentrations and cAMP-dependent protein kinase activity in particulate and soluble fractions of failing human hearts (ischaemic and dilated cardiomyopathy) and non-failing donor hearts.
Quantified phospholamban expression by Western blot with a monoclonal antibody and assessed its phosphorylation state using 32P-ATP as a substrate.
cAMP concentrations decreased in the particulate fraction of both ischaemic and dilated cardiomyopathy hearts, and decreased in the soluble fraction of dilated cardiomyopathy hearts.
cAMP-dependent protein kinase activity showed no detectable divergence between non-failing and failing myocardial tissue fractions.
Total phospholamban content and cAMP-dependent phospholamban phosphorylation remained unchanged between non-failing controls and failing hearts from either cardiomyopathy etiology.

Structured PICO

Population

Failing human hearts (ischaemic and dilated cardiomyopathy) and non-failing donor hearts

Comparator

Non-failing donor hearts

Outcome

cAMP concentrations, cAMP dependent protein kinase activity, and phospholamban levels and phosphorylationsurrogate

The reduction of cAMP formation is the predominant alteration in heart failure, while cAMP dependent protein kinase and phospholamban remain unchanged.

Cite This Study

Böhm et al. (1994) studied this question.

synapsesocial.com/papers/6a76949498f24dbf64bd3e7b https://doi.org/10.1093/cvr/28.11.1713

Topics

Women and heart disease

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Implication

Reduced cAMP predominates in HF without PKA or phospholamban changes; hypothesis-generating for upstream targeting, no practice implications.

cAMP concentrations, cAMP dependent protein kinase activity, and phospholamban in non-failing and failing myocardium

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Topics

cAMP concentrations, cAMP dependent protein kinase activity, and phospholamban in non-failing and failing myocardium

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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Topics

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