What does this research mean for the field?

Ca2+/calmodulin-dependent protein kinase (CaM kinase) activity is increased nearly three-fold in the hearts of patients with idiopathic dilated cardiomyopathy and correlates with hemodynamic dysfunction, whereas basal cAMP-dependent protein kinase (PKA) activity remains unchanged. Novelty: ClaimNovelty.INCREMENTAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

April 1, 1999Cardiovascular ResearchOpen Access

Activity of cAMP-dependent protein kinase and Ca2+/calmodulin-dependent protein kinase in failing and nonfailing human hearts

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Population

Left ventricular tissue of failing and nonfailing human hearts

Comparison

Measurement of cAMP-dependent protein kinase and… vs Nonfailing human hearts

Design

Preclinical

Authors

UKUwe KirchheferElectrophysiology

Discussion

Member takes

Implication

Hypothesis-generating for CaMKII-targeted therapy in dilated cardiomyopathy; requires human validation before clinical consideration.

Key Points

Evaluate the activity of cAMP-dependent protein kinase and Ca2+/calmodulin-dependent protein kinase in failing versus nonfailing human hearts.
Characterized PKA and CaM kinase activity using DEAE-Sepharose chromatography in heart tissues.
Measured activity in left ventricular tissue from patients with end-stage dilated cardiomyopathy and nonfailing hearts.
Basal PKA activity remained unchanged between failing and nonfailing hearts.
CaM kinase activity increased almost 3-fold in idiopathic dilated cardiomyopathy.
Hemodynamical parameters positively correlate with CaM kinase activity in patients with dilated cardiomyopathy.

Structured PICO

Population

Left ventricular tissue of failing (idiopathic dilated cardiomyopathy; ischemic heart disease) and nonfailing human hearts

Intervention

Measurement of cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin-dependent protein kinase II (CaM kinase) activity

Comparator

Nonfailing human hearts

Outcome

Activity of PKA and CaM kinasesurrogate

Increased CaM kinase activity in dilated cardiomyopathy may contribute to abnormal sarcoplasmic reticulum calcium handling in heart failure.

Cite This Study

Uwe Kirchhefer (1999) studied this question.

synapsesocial.com/papers/6a135f8071369867e4937d84 https://doi.org/10.1016/s0008-6363(98)00296-x

Topics

Heart failure HFrEF treatment

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Activity of cAMP-dependent protein kinase and Ca2+/calmodulin-dependent protein kinase in failing and nonfailing human hearts

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Topics

Activity of cAMP-dependent protein kinase and Ca2+/calmodulin-dependent protein kinase in failing and nonfailing human hearts

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Topics

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