What question did this study set out to answer?

To determine whether and at what stages of the cell cycle c-fos expression is required for fibroblast proliferation and DNA synthesis.

April 1, 1988Molecular and Cellular Biology

Microinjection of fos-Specific Antibodies Blocks DNA Synthesis in Fibroblast Cells

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Authors

KRKarl RiabowolUniversity of Calgary RVRobert J. VosatkaTufts University EZEdward B. ZiffNYU Langone Health

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Implication

In vitro study demonstrates that microinjecting fos-specific antibodies blocks DNA synthesis in fibroblasts, indicating c-fos activity is essential during early G1 cell cycle progression.

Key Points

To determine whether and at what stages of the cell cycle c-fos expression is required for fibroblast proliferation and DNA synthesis.
Microinjected affinity-purified anti-fos antibodies into quiescent REF-52 fibroblasts up to 6 to 8 hours following serum stimulation.
Introduced fos-specific antibodies or non-specific control immunoglobulins into asynchronously proliferating fibroblasts.
Microinjection of fos antibodies efficiently blocked serum-induced DNA synthesis when administered up to 6 to 8 hours post-stimulation in quiescent REF-52 fibroblasts.
Injection of fos antibodies into asynchronously growing cells prevented DNA synthesis in a consistently greater proportion of cells compared to non-specific immunoglobulin controls.
Results demonstrate that c-fos function is necessary both for the transition from G0 to G1 and during the early G1 phase to enable subsequent DNA replication.

Cite This Study

Riabowol et al. (1988) studied this question.

synapsesocial.com/papers/6a76b832576efb0ddaf6c637 https://doi.org/10.1128/mcb.8.4.1670-1676.1988

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Implication

In vitro study demonstrates that microinjecting fos-specific antibodies blocks DNA synthesis in fibroblasts, indicating c-fos activity is essential during early G1 cell cycle progression.

Microinjection of fos-Specific Antibodies Blocks DNA Synthesis in Fibroblast Cells

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Implication

Key Points

Cite This Study

Microinjection of fos-Specific Antibodies Blocks DNA Synthesis in Fibroblast Cells

Discussion

Member takes

Implication

Key Points

Cite This Study

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