Answers are grounded in this paper and cite their sources.
Fixed amphibian embryos and tadpoles were impregnated with acrylamide. After polymerization the gels were frozen and sectioned on a cryostat. The preservation of gross and fine morphological features in these sections is comparable to conventional paraffin sections and they can be stained by immunohistochemical methods without affecting the quality of the preparations.
No takes yet. Share an insight, caveat, or question.
Hausen et al. (1981) studied this question.
Synapse has enriched 10 related papers. Consider them for comparative context: