January 1, 2000The Journal of PhysiologyOpen Access

Substantial depletion of the intracellular Ca2+ stores is required for macroscopic activation of the Ca2+ release‐activated Ca2+ current in rat basophilic leukaemia cells

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Population

Rat basophilic leukaemia (RBL-1) cells

Comparison

Intracellular dialysis with Ca2+ mobilising… vs High cytoplasmic Ca2+ buffering conditions or…

Design

Preclinical

Authors

LFLeonardo FierroUniversidad Santiago de Cali APAnant B. ParekhNational Institute of Environmental Health Sciences

Discussion

Member takes

Implication

Threshold for store depletion in ICRAC activation is hypothesis-generating in RBL-1 cells; should not yet change clinical Ca2+ signaling practice.

Structured PICO

Population

Rat basophilic leukaemia (RBL-1) cells

Intervention

Intracellular dialysis with Ca2+ mobilising agents (IP3, ionomycin, thapsigargin, thapsigargicin, cyclopiazonic acid) under low cytoplasmic Ca2+ buffering conditions

Comparator

High cytoplasmic Ca2+ buffering conditions (10 mM EGTA) or different agent combinations

Outcome

Activation of macroscopic Ca2+ release-activated Ca2+ current (ICRAC)surrogate

Substantial depletion of intracellular Ca2+ stores below a specific threshold is required for the macroscopic activation of ICRAC in RBL-1 cells.

Cite This Study

Fierro et al. (2000) studied this question.

synapsesocial.com/papers/6a76dc2cd2fa2470761e7b84 https://doi.org/10.1111/j.1469-7793.2000.t01-1-00247.x

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Implication

Threshold for store depletion in ICRAC activation is hypothesis-generating in RBL-1 cells; should not yet change clinical Ca2+ signaling practice.

Substantial depletion of the intracellular Ca2+ stores is required for macroscopic activation of the Ca2+ release‐activated Ca2+ current in rat basophilic leukaemia cells

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Substantial depletion of the intracellular Ca2+ stores is required for macroscopic activation of the Ca2+ release‐activated Ca2+ current in rat basophilic leukaemia cells

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider