January 5, 1990Science

U1-Specific Protein C needed for Efficient Complex Formation of U1 snRNP with a 5′ Splice Site

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Population

U1 small nuclear ribonucleoproteins (snRNPs) and RNA molecules containing a 5' splice site sequence

Comparison

Depletion of U1-specific proteins C, A, and 70k… vs Intact U1 snRNPs or U1 snRNPs depleted of other…

Design

Preclinical

Authors

Discussion

Protein C supports U1 snRNP binding in this model; animal data leave open relevance to human splicing disorders.

Population

U1 small nuclear ribonucleoproteins (snRNPs) and RNA molecules containing a 5' splice site sequence

Intervention

Depletion of U1-specific proteins C, A, and 70k, and complementation with purified C protein

Comparator

Intact U1 snRNPs or U1 snRNPs depleted of other specific proteins

Outcome

Binding ability to an RNA molecule containing a 5' splice site sequence measured by nitrocellulose filter binding assay

Protein C is essential for the efficient formation of the U1 snRNP-pre-mRNA complex by potentiating base-pair interactions.

Heinrichs et al. (1990) studied this question.

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