January 1, 1990Nucleic Acids ResearchOpen Access

Crosslinking of hnRNP protiens to pre-mRNA requires U1 and U2 snRNPs

View Full Paper

Ask AI

Bookmark

Population

HeLa nuclear extract and in vitro transcribed beta-globin and adenovirus pre-mRNAs

Comparison

Oligodeoxynucleotide-directed RNase H cleavage… vs Untreated nuclear extract or extract treated…

Design

Preclinical

Authors

Discussion

HnRNP-pre-mRNA interactions require intact U1/U2 snRNPs; leaves open relevance to cardiac splicing regulation.

Population

HeLa nuclear extract and in vitro transcribed beta-globin and adenovirus pre-mRNAs

Intervention

Oligodeoxynucleotide-directed RNase H cleavage of U1 and U2 snRNAs

Comparator

Untreated nuclear extract or extract treated with control oligonucleotides (e.g., U5 oligo)

Outcome

Level of hnRNP protein crosslinking to pre-mRNA

The interaction of hnRNP proteins with pre-mRNA during early spliceosome assembly requires the presence of an intron and intact U1 and U2 snRNPs.

Mayrand et al. (1990) studied this question.

View Full Paper

Ask AI

Bookmark