What question did this study set out to answer?

To identify and characterize the specific surface binding sites and receptor mechanisms for bacterial lipopolysaccharides on human monocytes.

September 1, 1991The Journal of Immunology

Binding sites for endotoxins (lipopolysaccharides) on human monocytes

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Authors

CCChristine S. CouturierCentre National de la Recherche Scientifique NHNicole Haeffner‐CavaillonUniversité Paris-Sud MCMartine CaroffInria Saclay - Île de France

Discussion

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Implication

In vitro study uncovers multiple lipopolysaccharide binding mechanisms on human monocytes, indicating both CD14-dependent and independent pathways of bacterial endotoxin recognition.

Key Points

To identify and characterize the specific surface binding sites and receptor mechanisms for bacterial lipopolysaccharides on human monocytes.
Conducted radioligand binding assays using tritium-labeled intact lipopolysaccharides from Neisseria meningitidis and Salmonella minnesota R7, as well as a purified core region fragment (PS-OMe), in the presence and absence of serum.
Assessed receptor involvement via competitive binding assays using monoclonal antibodies against CD14 and CD11/CD18 complexes, alongside CD14-deficient monocytes isolated from patients with paroxysmal nocturnal hemoglobinuria.
Intact lipopolysaccharide exhibited saturable, serum-dependent binding directly mediated by CD14, which was inhibited by specific anti-CD14 monoclonal antibodies and absent on CD14-deficient monocytes, with no involvement of CD11/CD18 complexes.
The purified inner core region bound to an unidentified non-CD14 receptor site, while serum-free conditions exposed additional nonsaturable hydrophobic membrane interactions.

Cite This Study

Couturier et al. (1991) studied this question.

synapsesocial.com/papers/6a76f60498f24dbf64bd61ae https://doi.org/10.4049/jimmunol.147.6.1899

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September 1, 1991The Journal of Immunology

Binding sites for endotoxins (lipopolysaccharides) on human monocytes

View Full Paper

Ask AI

Bookmark

Authors

CCChristine S. CouturierCentre National de la Recherche Scientifique NHNicole Haeffner‐CavaillonUniversité Paris-Sud MCMartine CaroffInria Saclay - Île de France

Discussion

Member takes

Implication

In vitro study uncovers multiple lipopolysaccharide binding mechanisms on human monocytes, indicating both CD14-dependent and independent pathways of bacterial endotoxin recognition.

Key Points

To identify and characterize the specific surface binding sites and receptor mechanisms for bacterial lipopolysaccharides on human monocytes.
Conducted radioligand binding assays using tritium-labeled intact lipopolysaccharides from Neisseria meningitidis and Salmonella minnesota R7, as well as a purified core region fragment (PS-OMe), in the presence and absence of serum.
Assessed receptor involvement via competitive binding assays using monoclonal antibodies against CD14 and CD11/CD18 complexes, alongside CD14-deficient monocytes isolated from patients with paroxysmal nocturnal hemoglobinuria.
Intact lipopolysaccharide exhibited saturable, serum-dependent binding directly mediated by CD14, which was inhibited by specific anti-CD14 monoclonal antibodies and absent on CD14-deficient monocytes, with no involvement of CD11/CD18 complexes.
The purified inner core region bound to an unidentified non-CD14 receptor site, while serum-free conditions exposed additional nonsaturable hydrophobic membrane interactions.

Cite This Study

Couturier et al. (1991) studied this question.

synapsesocial.com/papers/6a76f60498f24dbf64bd61ae https://doi.org/10.4049/jimmunol.147.6.1899

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