Key result
E-4031 (10-100 nM) decreased the maximum upslope and peak amplitude of the field potential's second wave, whereas chromanol 293B did not, suggesting upslope decrease as a marker of IKr inhibition.
Why the study?
Does inhibition of IKr and IKs affect field-potential waveforms in human iPS cell-derived cardiomyocytes?
Does inhibition of IKr and IKs affect field-potential waveforms in human iPS cell-derived cardiomyocytes?
The decrease in the maximum upslope of the second wave of the field potential can serve as a supplemental marker for drug-induced IKr inhibition in human iPS cell-derived cardiomyocytes.
Answers are grounded in this paper and cite their sources.
May support field potential upslope as IKr marker in vitro; leaves open in vivo and clinical validation.
In order to investigate how IKr and IKs inhibitions affect waveforms of the field potential in the human iPS cell-derived cardiomyocytes sheet, we analyzed the effects of E-4031 and chromanol 293B on the maximum upslope and peak amplitude of its second wave (n = 7 for each drug). E-4031 in 10-100 nM as well as chromanol 293B in 3-30 μM prolonged the field-potential duration, whereas E-4031 decreased the upslope in 10-100 nM and amplitude at 100 nM, which was not observed by chromanol 293B. Thus, the decrease of the upslope can be used as a supplemental marker of drug-induced IKr inhibition.
No takes yet. Share an insight, caveat, or question.
Matsuo et al. (2015) studied human iPS cell-derived cardiomyocytes sheet (n=14). E-4031 and chromanol 293B was evaluated on maximum upslope and peak amplitude of the second wave of the field potential. E-4031 (10-100 nM) decreased the maximum upslope and peak amplitude of the field potential's second wave, whereas chromanol 293B did not, suggesting upslope decrease as a marker of IKr inhibition.
Synapse has enriched 10 related papers. Consider them for comparative context: