June 1, 1996Journal of VirologyOpen Access

A plasmid-based reverse genetics system for influenza A virus

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Population

human 293 cells

Comparison

plasmid-based expression vectors containing a… vs previously established in vitro-reconstituted…

Design

Preclinical

Authors

SPStephan PleschkaJustus-Liebig-Universität Gießen RJRichard JaskunasIcahn School of Medicine at Mount Sinai OEOthmar G. EngelhardtPotters Bar Community Hospital

Discussion

Member takes

Implication

May streamline recombinant influenza virus generation in labs; leaves open broader validation for pathogenesis or vaccine studies.

Structured PICO

Population

human 293 cells

Intervention

plasmid-based expression vectors containing a truncated human polymerase I (polI) promoter and a ribozyme sequence, cotransfected with polII-responsive plasmids expressing viral PB1, PB2, PA, and NP proteins

Comparator

previously established in vitro-reconstituted ribonucleoprotein (RNP) transfection system

Outcome

transcription and replication of the viral RNA-like reporter and rescue of a synthetic neuraminidase gene into a recombinant influenza virus

A novel plasmid-based reverse genetics system allows for efficient intracellular reconstitution of influenza A virus RNP complexes, providing a convenient alternative to RNP transfection.

Cite This Study

Pleschka et al. (1996) studied this question.

synapsesocial.com/papers/6a7724f39047b4aa92f6d187 https://doi.org/10.1128/jvi.70.6.4188-4192.1996

View Full Paper

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A plasmid-based reverse genetics system for influenza A virus

Discussion

Member takes

Implication

Structured PICO

Cite This Study

A plasmid-based reverse genetics system for influenza A virus

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider