A DNA transfection system for generation of influenza A virus from eight plasmids
Population
Cocultured 293T and MDCK cells
Design
Preclinical
Follow-up
72 hours
Authors
Discussion
Loading...
Member takes
Implication
Efficiently rescues reassortant influenza A viruses from cDNA; leaves open clinical translation and cardiovascular applications.
Structured PICO
P
Population
Cocultured 293T and MDCK cells
I
Intervention
Eight-plasmid DNA transfection system (pol I-pol II system) for the rescue of infectious influenza A virus from cloned cDNA
O
Outcome
Generation of infectious influenza A virus (virus yield)
The development of an eight-plasmid pol I-pol II transfection system enables the efficient rescue of infectious and reassortant influenza A viruses entirely from cloned cDNA.
Cite This Study
Hoffmann et al. (2000) studied this question.