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June 15, 1991Proceedings of the National Academy of SciencesOpen Access

In vivo analysis of the promoter structure of the influenza virus RNA genome using a transfection system with an engineered RNA.

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Key result

Transfection of HeLa cells with antisense CAT RNA complexed with purified nucleoprotein and helper ribonucleoprotein cores led to efficient expression of the CAT gene.

Population

HeLa cells

Comparison

Transfection with antisense chloramphenicol… vs Transfection with naked antisense CAT RNA or RNP…

Design

Preclinical

Authors

KYK YamanakaThe University of OsakaNONaotaka OgasawaraAichi Medical UniversityHYHiroki YoshikawaNihon University

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Implication

Enables foreign gene expression in negative-strand RNA viruses; extends foundational tools for virology research.

Structured PICO

P
Population
HeLa cells
I
Intervention
Transfection with antisense chloramphenicol acetyltransferase (CAT) RNA covered with purified nucleoprotein (RNA-NP complexes) and helper ribonucleoprotein (RNP) cores
C
Comparator
Transfection with naked antisense CAT RNA or RNP cores alone
O
Outcome
Expression of the chloramphenicol acetyltransferase (CAT) gene (detected by immunostaining and CAT mRNA by PCR)surrogate

The study developed an in vivo transcription system for negative-polarity RNA using influenza virus components, revealing that critical promoter signals are located at positions 6-14 from the 3' end of the viral RNA genome.

Cite This Study

Yamanaka et al. (1991) studied this question. Antisense CAT RNA-NP complexes vs. Naked RNA or RNP cores alone was evaluated on CAT gene expression. Transfection of HeLa cells with antisense CAT RNA complexed with purified nucleoprotein and helper ribonucleoprotein cores led to efficient expression of the CAT gene.

synapsesocial.com/papers/6a6e82a8e5469ee92be003fdhttps://doi.org/10.1073/pnas.88.12.5369
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Also Consider

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  3. 3In vitro synthesis of an infectious RNA from cDNA clones of human rhinovirus type 141985 · 88 citations
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  5. 5The use of DNA-cellulose for analyzing histone-DNA interactions. Discovery of nucleosome-like histone binding to single-stranded DNA.1979 · 41 citations