June 1, 1991Journal of VirologyOpen Access

The polyadenylation signal of influenza virus RNA involves a stretch of uridines followed by the RNA duplex of the panhandle structure

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Population

in vitro-reconstituted RNA-protein complexes and cells transfected with plasmid-derived RNA containing CAT…

Comparison

Mutations introduced into both the 5' and 3'… vs Wild-type RNA or other mutant RNAs

Design

Preclinical

Authors

GLGaoxing LuoSouthwest General Health Center WLWillem LuytjesNational Institute for Public Health and the Environment MEMasayoshi EnamiKanazawa University

Discussion

Member takes

Implication

Offers no immediate clinical implications; extends mechanistic understanding of influenza polymerase in animal models.

Structured PICO

Population

in vitro-reconstituted RNA-protein complexes and cells transfected with plasmid-derived RNA containing CAT reporter gene flanked by NS RNA segment of influenza A/WSN/33 virus

Intervention

Mutations introduced into both the 5' and 3' ends of the RNA

Comparator

Wild-type RNA or other mutant RNAs

Outcome

Transcription in vitro and CAT expression activitysurrogate

The study demonstrates that influenza viral RNA polymerase adds poly(A) by a slippage mechanism when it encounters a double-stranded RNA barrier next to a stretch of uridines.

Cite This Study

Luo et al. (1991) studied this question.

synapsesocial.com/papers/6a7c548bdb925e97554008fc https://doi.org/10.1128/jvi.65.6.2861-2867.1991

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The polyadenylation signal of influenza virus RNA involves a stretch of uridines followed by the RNA duplex of the panhandle structure

Discussion

Member takes

Implication

Structured PICO

Cite This Study

The polyadenylation signal of influenza virus RNA involves a stretch of uridines followed by the RNA duplex of the panhandle structure

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider