What question did this study set out to answer?

To determine whether human pulmonary alveolar macrophages are susceptible to HTLV-III infection and evaluate their potential to act as a viral reservoir.

July 1, 1986BloodOpen Access

Human T lymphotropic virus type III infection of human alveolar macrophages

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Population

Human pulmonary macrophages in vitro, and primary cultures of pulmonary macrophages from 4 patients with AIDS

Design

Preclinical

Follow-up

up to 2 weeks (in vitro)

Authors

SSSZ SalahuddinNational Institutes of Health RRR. M. RoseHarvard University JGJE GroopmanBeth Israel Deaconess Medical Center

Discussion

Member takes

Implication

Suggests alveolar macrophages as potential HIV reservoirs; leaves open their role in persistence and transmission pending in vivo confirmation.

Key Points

To determine whether human pulmonary alveolar macrophages are susceptible to HTLV-III infection and evaluate their potential to act as a viral reservoir.
Exposed cultured human pulmonary alveolar macrophages in vitro to HTLV-III and assessed viral transmission to allogeneic peripheral blood mononuclear leukocytes over two weeks.
Cultured primary pulmonary macrophages isolated from four patients with AIDS (N=4) to measure spontaneous viral production via precipitable reverse transcriptase activity.
In vitro HTLV-III-infected alveolar macrophages produced low virus levels and successfully transferred infection to peripheral blood mononuclear leukocytes up to two weeks post-infection while resisting viral cytopathology.
Primary alveolar macrophage cultures from two of four patients with AIDS spontaneously generated detectable reverse transcriptase activity, confirming in vivo infection.

Structured PICO

Population

Human pulmonary (alveolar) macrophages in vitro, and primary cultures of pulmonary macrophages from 4 patients with AIDS

Intervention

HTLV-III infection

Outcome

Susceptibility to infection and viral production

Human pulmonary macrophages can be infected by HTLV-III and may act as a long-lived viral reservoir capable of transmitting the virus to T lymphocytes.

Cite This Study

Salahuddin et al. (1986) studied this question.

synapsesocial.com/papers/6a772a5af6b553d308ae4e8c https://doi.org/10.1182/blood.v68.1.281.281

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Implication

Suggests alveolar macrophages as potential HIV reservoirs; leaves open their role in persistence and transmission pending in vivo confirmation.

Human T lymphotropic virus type III infection of human alveolar macrophages

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Human T lymphotropic virus type III infection of human alveolar macrophages

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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