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May 18, 2012PLoS ONEOpen Access

Atomic Force Mechanobiology of Pluripotent Stem Cell-Derived Cardiomyocytes

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Key result

Cardiomyocytes derived from subjects with dilated cardiomyopathy showed significantly decreased contraction force (0.55 nN vs 1.35 nN) and cellular elasticity compared to healthy controls.

Population

Pluripotent stem cell-derived cardiomyocytes, including human embryonic stem cell line H7 and skin…

Comparison

Atomic force microscopy to quantify… vs Healthy control iPSC-CMs and pre-treatment…

Design

Preclinical

Authors

JLJianwei LiuTianjin University of Traditional Chinese MedicineNSNing SunJiangnan UniversityMBMarc A. BruceUniversity of Central Florida

Discussion

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Implication

Hypothesis-generating for iPSC-cardiomyocyte models in dilated cardiomyopathy; should not change practice without human validation.

Structured PICO

P
Population
In vitro study of cardiomyocytes derived from induced pluripotent stem cells of healthy subjects and patients with dilated cardiomyopathy, as well as embryonic stem cell lines.
E
Exposure
Atomic force microscopy (AFM) to quantify mechanobiological properties, along with pharmacological testing using norepinephrine, epinephrine, and metoprolol.
C
Comparator
Healthy control iPSC-CMs (for DCM comparison) and pre-treatment baseline (for pharmacological effects).
O
Outcome
Mechanobiological properties including contraction force, beat rate, beat duration, and cellular elasticity (Young's modulus).surrogate

Main Result

Absolute Event Rate: 0.55% vs 1.35%

p-value: p=<10^-216

Atomic force microscopy can successfully quantify the mechanobiological properties of stem cell-derived cardiomyocytes, demonstrating reduced force and elasticity in dilated cardiomyopathy and dose-responsive inotropic effects to adrenergic stimulation.

Limitations

  • Total force output may be higher than measured due to unmeasured lateral modes of contraction.
  • Stress relaxation in elasticity measurements of live cells due to cytoskeletal reorganization.

Cite This Study

Liu et al. (2012) studied Dilated Cardiomyopathy. Dilated cardiomyopathy (DCM) mutation vs. Healthy control cells was evaluated on Contraction force (nN) (p=<10^-216). Cardiomyocytes derived from subjects with dilated cardiomyopathy showed significantly decreased contraction force (0.55 nN vs 1.35 nN) and cellular elasticity compared to healthy controls.

synapsesocial.com/papers/6a7733703da069e66cbd8a69https://doi.org/10.1371/journal.pone.0037559
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Contemporary Definitions and Classification of the Cardiomyopathies2006 · 3,465 citations
  2. 2Patient-Specific Induced Pluripotent Stem Cells as a Model for Familial Dilated Cardiomyopathy2012 · 706 citations
  3. 3Progress With Genetic Cardiomyopathies2009 · 217 citations
  4. 4Calibration of atomic-force microscope tips1993 · 4,248 citations
  5. 5Control of cardiac muscle cell function by an endogenous nitric oxide signaling system.1993 · 662 citations