EFFECTS OF ERYTHROPOIETIN AND Hypericum Perforatum ON GENTAMICIN-INDUCED AGT, β-catenin, iNOS AND eNOS IMMUNOREACTIVITIES

Gentamicin (GM) is an antibiotic used in the treatment of acute infections caused by gram-negative microorganisms.Erythropoietin (EPO) is a cytokine that regulates cell proliferation and differentiation and has physiological roles in tissue protection.Hypericum perforatum (HP) is a phytochemical antioxidant with free radical scavenging and cell rejenerative properties.This study aimed to examine the effects of EPO and HP on GM-induced angiotensinogen (AGT), β-catenin, inducible nitric oxide synthase (iNOS) and endothelial nitric oxide synthase (eNOS) immunoreactivities.36 male Spraque-Dawley rats were divided into control, GM, GM+EPO, GM+HP, EPO and HP groups.It was determined that EPO and HP decreased the GMinduced increased AGT, β-catenin, iNOS immunoreactivities and increased the decreased eNOS immunoreactivity.In conclusion, it is thought that the renoprotective effects of EPO and HP can regulate the immunohistochemical changes caused by GM in the kidney.