An attempt was made to clarify the effect of culture conditions of an acetic acid bacteria ( Acetobacter xylinum ) on cellulose biosynthesis using glucose as carbon source in complex medium. Synchronous culture conditions were first realised on cellulose biosynthesis by cooling the system to 4°C for 24 h. Under the synchronous conditions stepwise division of the cell and the stepwise production of cellulose were found. Furthermore, cellulose was proved to be produced when the cell number in the medium was constant.
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Kamide et al. (1990) studied this question.
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