Oxidized LDL Increase the Proinflammatory Profile of Human Visceral Adipocytes Produced by Hypoxia

Figures

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  Figure 1. (A) Levels of mRNA expression of HIF-1α, TNFα and IL6 in human visceral adipocytes from non-obese subjects and patients with morbid obesity (MO). Data are the mean ± SEM. * p < 0.05: significant differences between non-obese subjects and MO. (B) Significant correlation found in visceral mature adipocytes between the mRNA expression of HIF-1α and IL6.

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  Figure 2. Levels of mRNA expression of scavenger receptors in human visceral adipocytes from non-obese subjects and patients with morbid obesity (MO). Data are the mean ± SEM. * p < 0.05: significant differences between non-obese subjects and MO.

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  Figure 3. Significant correlations found in visceral mature adipocytes between the mRNA expression of scavenger receptors with HIF-1α and IL6.

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  Figure 4. Significant correlations found in visceral mature adipocytes between the mRNA expression of HIF-1α and scavenger receptors.

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  Figure 5. Levels of HIF-1α, GLUT1, IL6, TNFα and scavenger receptors mRNA expression of in vitro-differentiated adipocytes obtained from human mesenchymal stem cells of visceral adipose tissue (VAT) from non-obese subjects (n = 5) and patients with morbid obesity (n = 5) (MO) incubated in normoxia (■) and hypoxia (□) conditions for 24 h. Each treatment was performed in triplicate. Data are the mean ± SEM. * p < 0.05: significant differences between the normoxia and hypoxia conditions.

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  Figure 6. Levels of HIF-1α, GLUT1, TNFα, IL6 and scavenger receptors mRNA expression (%) of in vitro-differentiated adipocytes obtained from human mesenchymal stem cells of visceral adipose tissue (VAT) from non-obese subjects (n = 5) incubated in normoxia and hypoxia conditions when HIF-1α was silenced as described in the Materials and Methods section. Briefly, at day 15 of adipocyte differentiation, the transfection was made with a siRNA (s6539) and DharmaFECTTM Transfection Reagent. After 24 h in the normoxia condition, the cells were incubated for another 24 h in normoxia or hypoxia conditions. Cells were harvested and frozen at −80 °C until analysis. Each treatment was performed in triplicate. Data are the mean ± SEM. Results are shown as a percentage with regard to normoxia condition (100%) without hypoxia and HIF-1α silencing. * p < 0.05: significant differences between silenced and not silenced HIF-1α conditions. 1 p < 0.05: significant differences between normoxia and hypoxia conditions when HIF-1α is or is not silenced.

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  Figure 7. Levels of mRNA expression (%) of TNFα and IL6 of in vitro-differentiated adipocytes obtained from human mesenchymal stem cells of VAT from non-obese subjects (n = 5) incubated in hypoxia conditions with ox-LDL. Briefly, at day 15 of adipocyte differentiation, the cells were incubated for 48 h in normoxia or hypoxia conditions. After the first 24 h of incubation, 0 and 50 µg/mL of human ox-LDL were added. After another 24 h of incubation, the adipocytes were harvested and frozen at −80 °C until analysis. Each treatment was performed in triplicate. Data are the mean ± SEM. . Results are shown as a percentage with regard to normoxia condition (100%) without hypoxia and ox-LDL. a p < 0.05: significant differences respect to the normoxia condition without 50 µg/mL ox-LDL. 1 p < 0.05: significant differences between hypoxia and hypoxia + ox-LDL conditions.