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August 9, 2026VirusesOpen Access

Isolation, Characterization and Reverse Genetic System Establishment of a Highly Virulent PEDV Strain

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Authors

FZFan ZhangHLHelu Liu继龙继林 龙

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Overview

Randomized trial demonstrates viral pathogenesis in piglets, highlighting the need for effective PEDV vaccines.

Key Points

  • The aim is to isolate and characterize a virulent PEDV strain and establish a reverse genetics system for vaccine development.
  • Isolated PEDV-BJ-2023 from diarrheic piglets in Guizhou, China.
  • Constructed a full-length infectious cDNA clone using transformation-associated recombination.
  • Generated an enhanced green fluorescent protein reporter virus via CRISPR/Cas9 homologous recombination.
  • All three tested viruses caused severe diarrhea, weight loss, and intestinal lesions in 5-day-old piglets.
  • Recombinant viruses showed slightly reduced pathogenicity with rPEDV-EGFP being the most attenuated.
  • rPEDV-EGFP maintained stable EGFP expression over eight serial passages.

Cite This Study

Zhang et al. (2026) studied this question.

synapsesocial.com/papers/6a782d2c2e1896536c84002dhttps://doi.org/10.3390/v18080864
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Also Consider

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  1. 1Emergence of a Highly Virulent Porcine Epidemic Diarrhea Virus (PEDV) G2c Subtype in China: Isolation, Genetic and Pathogenic Characterization, and Cross‐Neutralizing Antibody Response2026 · 7 citations
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