Monoamine Oxidase-Dependent Pro-Survival Signaling in Diabetic Hearts Is Mediated by miRNAs

Figures

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  Figure 1. mRNA gene expression. (A) Dendrogram describing relationship of samples obtained according to mRNA gene expression. (B) Clusters of differentially expressed genes. Gene expression was calculated in relation to the average expression of the gene in all samples. (C) Venn diagram describing the number of differentially expressed mRNA for each comparison. (D) Validation of the mRNA microarray through qRT-PCR. mRNA levels were normalized to the housekeeping gene Tbp. * p ≤ 0.05 vs. C. C: control; C + P: control + pargyline; D: diabetes; D + P: diabetes + pargyline; Anp: atrial natriuretic peptide; Pdk4: pyruvate dehydrogenase kinase 4; Prdx4: peroxiredoxin 4; Txnip: thioredoxin interacting protein; Angptl4: angiopoietin-like 4; Rgs2: regulator of G-protein signaling 2.

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  Figure 2. Effects of MAO inhibition on autophagy flux in T1D hearts and NRVMs cultured with high glucose. (A) Representative Western blot (upper panel) and densitometry analyses (lower panel) of the autophagy marker LC3B-II in control (C), diabetic (D) and diabetic mice treated with the MAO inhibitor pargyline (D + P). Values were normalized to actin or Red Ponceau staining. * p ≤ 0.05 vs. C; # p ≤ 0.05 vs. D, by Dunn’s non-parametric test. (B) Representative Western blots (upper panel) and densitometry analyses (lower panel) of LC3B-II in NRVMs cultured with the osmotic control mannitol (HM) or high glucose (HG) at baseline or after treatment with the inhibitor of lysosomal degradation chloroquine (chloro). Parallel experiments were performed in the presence of MAO inhibitor pargyline. Values were normalized to actin. LC3B-II abundance in HM groups was arbitrarily considered as a unit. * p ≤ 0.05 vs. HG chloro, with two-tailed equal variance t-test.

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  Figure 3. MiRNA gene expression. Heatmap of differentially expressed miRNAs. Both samples and miRNAs were clustered according to average dot products and complete linkage. Cluster 1 represents miRNAs upregulated in heart samples of diabetic mice that returned to control levels after pargyline treatment. Cluster 2 represents miRNAs downregulated in heart samples of diabetic mice that were normalized after pargyline treatment. C: control; C + P: control + pargyline; D: diabetes; D + P: diabetes + pargyline.

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  Figure 4. Transcriptomic miRNA expression validation. MiRNA expression was calculated through qRT-PCR relative to U6. Error bars indicate standard deviation calculated on at least three samples and three technical replicates per sample. Significance was calculated using a t-test between samples considering unequal variance between samples. * p ≤ 0.05; ** p ≤ 0.002. C: control; C + P: control + pargyline; D: diabetes; D + P: diabetes + pargyline.

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  Figure 5. MiRNAs expression after MAO-A downregulation. MiRNAs expression was calculated through qRT-PCR relative to U6. Error bars indicate standard deviation calculated on at least three samples and three technical replicates per sample. Significance was calculated using t-test between samples considering unequal variance between samples. * p ≤ 0.05 vs. HM scramble; # p ≤ 0.05 vs. HG scramble.

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  Figure 6. Interaction of miRNAs with their targets. (A) Network representing the interaction between miRNA (triangles) and targets (rectangles). Interactions among rectangles represent interactions retrieved from the BioGrid database. Colors inside nodes represent gene expression calculated as D/D + P, while border colors represent gene expression calculated as D/C. Green arrows indicate target miRNAs for which we validated miRNA–gene interactions. (B) Luciferase assay was performed to demonstrate the direct interaction between miR-133a-3p and Igf1r; miR-27a-3p and Inpp4a, Elk1 and Rps6ka2; miR-193a-3p and Mapk10 and Grb10. Part of Igf1r, Inpp4a, Elk1, Rps6ka2, Mapk10 and Grb10 sequences containing putative miRNA interaction sites (or not containing; Igf1r-, Inpp4a-, Elk1-, Rps6ka2-, Mapk10-, Grb10-Ctrl) were cloned into pmirGLO vector. Firefly luciferase (reporter gene) and Renilla luciferase (control reporter for normalization) activities were measured after transfection of cardiomyocytes together with miRNA mimics or a scramble sequence (Ctrl). (C) Gene expression of miR-27a-3p and -193a-3p targets after specific miRNA or scramble overexpression in cardiomyocytes. For both panels, data…

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  Figure 7. Effect of MAO inhibition on AKT activation in diabetic hearts. (A) Representative Western blot (upper panel) and densitometry analyses (lower panel) of the AKT phosphorylated on Thr308 and total AKT are shown in control (C), diabetic (D) and diabetic mice treated with the MAO inhibitor pargyline (D + P). Values were normalized to total AKT levels. * p ≤ 0.05 vs. C, # p ≤ 0.05 vs. D, with one-tailed equal variance t-test. (B) Representative Western blot (upper panel) and densitometry analyses (lower panel) of the AKT phosphorylated on Ser473 and total AKT are shown in control (C), diabetic (D) and diabetic mice treated with the MAO inhibitor pargyline (D + P). Values were normalized to total AKT levels. * p ≤ 0.05 vs. C, with one-tailed equal variance t-test.

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  Figure 8. Graphical abstract of the main findings of this study indicating MAO-dependent post-transcriptional regulation of Igf1r, Grb10 and Inpp4a via miR-133a-3p, -27a-3p and -193a-3p, which in turn regulate pro-survival signaling through AKT activation in diabetic hearts.