Ticagrelor Can Regulate the Ion Channel Characteristics of Superior Cervical Ganglion Neurons after Myocardial Infarction

Figures

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  Figure 1. MI model validation and changes in cardiac function after MI and TIC. (A) ECG of MI group; (B) CK and CKMB in serum in control and MI groups; (C) sample echocardiography images for the three groups; (D) histograms of LAD, LVDD, LVEF, and FS in the three groups. MI, myocardiac infarction; TIC, ticagrelor; LAD, left atrial diameter; LVDD, left ventricular end-diastolic dimension; LVEF, left ventricular ejection fraction; FS, fractional shortening. Each point represents the mean ± SD, * p < 0.05 compared with the control group. # p < 0.05 compared with the MI group.

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  Figure 2. Expression of P2Y12 in SCGs after MI and TIC. (A) P2Y12 and TH distributions for the three groups were determined by immunofluorescence staining; (B) P2Y12 expression in the three groups was determined by RT-PCR; (C) the NE content in the serum of the three groups. SCG, superior cervical ganglion; MI, myocardiac infarction; TIC, ticagrelor; TH, tyrosine hydroxylase. Each point represents the mean ± SD, * p < 0.05 compared with the control group. # p < 0.05 compared with the MI group.

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  Figure 3. Effects of MI and TIC on IK characteristics. (A) The patch-clamp protocol and typical examples of IK; (B) I-V curves for IK for the three groups; (C) the patch-clamp protocol and activation curves for IK in the three groups. MI, myocardiac infarction; TIC, ticagrelor. Each point represents the mean ± SD, * p < 0.05 compared with the control group. # p < 0.05 compared with the MI group.

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  Figure 4. Effects of MI and TIC on INa characteristics. (A) The patch-clamp protocol and typical examples of INa; (B) I-V curves for INa for the three groups; (C) the patch-clamp protocol and activation curves for INa for the three groups; (D) the patch-clamp protocol and inactivation curves for INa for the three groups; (E) the patch-clamp protocol and recovery-from-inactivation curves for INa for the three groups. MI, myocardiac infarction; TIC, ticagrelor. Each point represents the mean ± SD, * p < 0.05 compared with the control group. # p < 0.05 compared with the MI group.

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  Figure 5. Effects of MI and TIC on ICa and intracellular calcium concentration. (A) The patch-clamp protocol and typical examples of ICa; (B) I-V curves for ICa for the three groups; (C) representative recordings of fluorescence curves for intracellular calcium concentration. (D) Ratio of peak to resting fluorescence (F/F0 ratios) for the three groups. MI, myocardiac infarction; TIC, ticagrelor. Each point represents the mean ± SD, * p < 0.05 compared with the control group.