Key result
Affimers 6, 14, and 24 bind tightly to F-actin with Kds of ~0.3 μM and successfully label F-actin in live and methanol-fixed cells, offering a novel alternative to phalloidin.
Population
In vitro purified F-actin, live cells (Hela, COS-7, B16), and fixed cells (Hela, Sf9)
Comparison
Affimers as eGFP-fusion proteins or biotinylated… vs Phalloidin, Lifeact, and F-tractin
Design
Preclinical
Authors
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Affimers expand live-cell F-actin imaging options; leaves open validation in cardiovascular cell models.
Affimers 6, 14, and 24 are novel affinity reagents that can effectively label F-actin in live and fixed cells, offering an alternative to phalloidin, Lifeact, and F-tractin.
Lopata et al. (2018) studied this question. Affimer proteins (Affimers 6, 14, and 24) vs. Phalloidin, Lifeact, F-tractin was evaluated on Binding affinity (Kd) to F-actin and cellular labeling. Affimers 6, 14, and 24 bind tightly to F-actin with Kds of ~0.3 μM and successfully label F-actin in live and methanol-fixed cells, offering a novel alternative to phalloidin.
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