Key result
Dual labeling of cells with rhodamine DNAse I and FITC-phalloidin provided a sensitive and direct method to image and quantify actin rearrangement in individual cells.
Population
Attached or suspended fibroblasts
Design
Preclinical
Authors
Loading...
Enables actin imaging in animal cells; leaves open validation for human cardiovascular research.
Dual labeling of cells with rhodamine DNAse I and FITC-phalloidin provides a simple and direct method to image and quantify actin rearrangement in individual cells.
Knowles et al. (1992) studied this question. Dual labeling with rhodamine DNAse I and FITC-phalloidin was evaluated on Localization and quantification of relative G and F actin content. Dual labeling of cells with rhodamine DNAse I and FITC-phalloidin provided a sensitive and direct method to image and quantify actin rearrangement in individual cells.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: