Key result
Replacement of ATP in Mg-G-actin by ADP caused a ~70% increase in quantum yield and a 12 nm blue shift in emission spectrum without changing the FRET distance between donor and acceptor probes.
Population
G-actin (Ca-ATP-G-actin conjugated with dansyl cadaverine at Gln41 and DDPM at Cys374)
Comparison
DNase I binding, Ca2+/Mg2+ exchange, ATP/ADP… vs Baseline/unbound states
Design
Preclinical
Authors
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Should not change clinical practice; extends G-actin conformational models but leaves open cardiac isoform relevance.
Fluorescence spectroscopy reveals conformational changes in subdomain 2 of G-actin upon nucleotide exchange and proteolytic cleavage, but not upon DNase I binding.
Moraczewska et al. (1996) studied this question. DNase I binding, Ca2+/Mg2+ and ATP/ADP exchange was evaluated on Fluorescence of dansyl cadaverine (DC) conjugated to Gln41 and FRET distance. Replacement of ATP in Mg-G-actin by ADP caused a ~70% increase in quantum yield and a 12 nm blue shift in emission spectrum without changing the FRET distance between donor and acceptor probes.
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