May 1, 1981Proceedings of the National Academy of SciencesOpen Access

Differential staining of actin in metaphase spindles with 7-nitrobenz-2-oxa-1,3-diazole-phallacidin and fluorescent DNase: is actin involved in chromosomal movement?

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Population

Rat kangaroo cells (PtK2)

Design

Preclinical

Authors

LBLawrence S BarakDuke University ENEugene A. NothnagelUniversity of California, Riverside EDE F DeMarcoCornell University

Discussion

Member takes

Implication

Excludes F-actin–dependent spindle models; leaves open G-actin contributions to chromosome movement pending mammalian validation.

Structured PICO

Population

Rat kangaroo cells (PtK2)

Intervention

Staining with 7-nitrobenz-2-oxa-1,3-diazole-phallacidin (NBD-Ph) and fluorescent DNase I (Rh-DNase or Fl-DNase)

Outcome

Distribution and polymerization state of actin in metaphase spindlessurrogate

The study demonstrates that the metaphase spindle contains G actin but lacks significant F actin, challenging models of chromosomal movement that rely on extensive actin sliding filaments within the spindle.

Limitations

Cannot exclude the possibility that the spindle contains F actin in amounts too small to be detected by fluorescence microscopy (e.g., fewer than five filaments per chromosome)

Cite This Study

Barak et al. (1981) studied this question.

synapsesocial.com/papers/6a79de62e357fbdac589410c https://doi.org/10.1073/pnas.78.5.3034

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Differential staining of actin in metaphase spindles with 7-nitrobenz-2-oxa-1,3-diazole-phallacidin and fluorescent DNase: is actin involved in chromosomal movement?

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

Differential staining of actin in metaphase spindles with 7-nitrobenz-2-oxa-1,3-diazole-phallacidin and fluorescent DNase: is actin involved in chromosomal movement?

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

Also Consider

Also Consider