What question did this study set out to answer?

To determine how partial sodium pump inhibition and sodium-hydrogen exchange inhibition affect intracellular pH, calcium concentrations, and contractility during acidosis.

November 1, 1991AJP Cell Physiology

Effect of partial Na pump and Na-H exchange inhibition on Cai during acidosis in cardiac cells

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Why the study?

Does partial Na pump inhibition and Na-H exchange inhibition alter intracellular Ca concentration and contractile function during acidosis in isolated rabbit cardiac cells?

Population

Isolated cardiac cells of adult rabbits

Comparison

Partial Na pump inhibition with ouabain and/or… vs Absence of ouabain or EIPA during intracellular…

Design

Preclinical

Authors

TNToshio NakanishiTokyo Women's Medical University MSM. SeguchiSeirei Hamamatsu General Hospital TTT. TsuchiyaKai Research (United States)

Discussion

Member takes

Implication

In adult rabbit myocytes, Na pump inhibition enhances the increase in intracellular calcium during acidosis, while Na-H exchange inhibition reduces it, suggesting a role for altered Na-Ca exchange.

Key Points

To determine how partial sodium pump inhibition and sodium-hydrogen exchange inhibition affect intracellular pH, calcium concentrations, and contractility during acidosis.
Isolated ventricular myocytes from adult rabbits and measured intracellular pH with BCECF and intracellular calcium with fura-2.
Induced intracellular acidosis under normal extracellular pH using an ammonium chloride (10 mM) prepulse technique.
Treated cells with 0.5 µM ouabain to partially inhibit the sodium pump and with EIPA to inhibit sodium-hydrogen exchange.
Ouabain enhanced both systolic and diastolic intracellular calcium increases and improved contractile recovery during acidosis without altering intracellular pH recovery.
EIPA blunted intracellular pH recovery, contractile function recovery, and the rise in intracellular calcium both in the presence and absence of ouabain.

Structured PICO

Does partial Na pump inhibition and Na-H exchange inhibition alter intracellular Ca concentration and contractile function during acidosis in isolated rabbit cardiac cells?

Population

Isolated cardiac cells of adult rabbits

Intervention

Partial Na pump inhibition with ouabain (0.5 microM) and/or Na-H exchange inhibition with 5-(N-ethyl-N-isopropyl)amiloride (EIPA) during intracellular acidosis induced by NH4Cl (10 mM) prepulse

Comparator

Absence of ouabain or EIPA during intracellular acidosis

Outcome

Intracellular Ca concentration ([Ca]i), intracellular pH (pHi), and contractile functionsurrogate

In adult rabbit myocytes, Na pump inhibition enhances the increase in intracellular calcium during acidosis, while Na-H exchange inhibition reduces it, suggesting a role for altered Na-Ca exchange.

Cite This Study

Nakanishi et al. (1991) studied this question.

synapsesocial.com/papers/6a7aa30b06bbdce361021f37 https://doi.org/10.1152/ajpcell.1991.261.5.c758

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Implication

In adult rabbit myocytes, Na pump inhibition enhances the increase in intracellular calcium during acidosis, while Na-H exchange inhibition reduces it, suggesting a role for altered Na-Ca exchange.

Effect of partial Na pump and Na-H exchange inhibition on Cai during acidosis in cardiac cells

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Effect of partial Na pump and Na-H exchange inhibition on Cai during acidosis in cardiac cells

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider