January 1, 1996British Journal of PharmacologyOpen Access

Mechanisms of tolerance to sodium nitroprusside in rat cultured aortic smooth muscle cells

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Population

Rat cultured aortic smooth muscle cells

Comparison

Pretreatment with sodium nitroprusside 10-500… vs Untreated cells, or co-incubation with…

Design

Preclinical

Authors

APAndreas PapapetropoulosUniversity of Siena CGCarolyn Y. Go FMFerid MuradCross-Cutting Cardiology

Discussion

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Implication

May inform SNP tolerance mechanisms in vascular research; leaves open any clinical relevance pending human validation.

Structured PICO

Population

Rat cultured aortic smooth muscle cells

Intervention

Pretreatment with sodium nitroprusside (SNP) 10-500 microM, zaprinast, S-nitroso N-acetylpenicillamine (SNAP), or 8Br-cyclic GMP

Comparator

Untreated cells, or co-incubation with oxyhaemoglobin, H89 (PKA inhibitor), or reducing agents

Outcome

Soluble guanylate cyclase (sGC) activity estimated from the accumulation of cyclic GMP in response to 10 microM SNP over a 15 min period in the presence of a phosphodiesterase (PDE) inhibitorsurrogate

Tolerance to sodium nitroprusside in rat aortic smooth muscle cells is driven by both an early NO-mediated mechanism and a delayed PKA-sensitive process involving decreased sGC protein levels.

Cite This Study

Papapetropoulos et al. (1996) studied this question.

synapsesocial.com/papers/6a7ac17d14b028facf0e889e https://doi.org/10.1111/j.1476-5381.1996.tb15167.x

View Full Paper

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Mechanisms of tolerance to sodium nitroprusside in rat cultured aortic smooth muscle cells

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Mechanisms of tolerance to sodium nitroprusside in rat cultured aortic smooth muscle cells

Discussion

Member takes

Implication

Structured PICO

Cite This Study

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Also Consider