Why the study?
EV-A71 is primarily transmitted through the fecal-oral route, creating a need for suitable animal models of oral infection to investigate viral pathogenesis.
A novel oral infection model in 21-day-old hSCARB2 transgenic mice successfully replicates severe EV-A71 pathogenesis and allows for real-time tracking of viral spread.
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May facilitate preclinical EV-A71 antiviral testing; leaves open human translation from this transgenic mouse model.
, poses a persistent global public health problem. EV-A71 is primarily transmitted through the fecal-oral route, and thus suitable animal models of oral infection are needed to investigate viral pathogenesis. We present an animal model of EV-A71 infection that enables the natural route of oral infection in weaned and nonimmunocompromised 21-day-old hSCARB2 transgenic mice. Our results demonstrate that severe disease and death could be stably induced, and viral invasion of the CNS could be replicated in this model, similar to severe real-world EV-A71 infections. We also developed a nanoluciferase-containing EV-A71 virus that can be used with this animal model to track viral spread after oral infection in real time. Such a model offers several advantages over existing animal models and can facilitate future research into viral spread, tissue tropism, and viral pathogenesis, all pressing issues that remain unaddressed for EV-A71 infections.
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Lin et al. (2021) studied this question.
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