Key result
T2-prepared imaging provided reproducible T2 maps of mouse myocardium, with infarct T2 significantly higher than remote tissue and infarct sizes strongly correlating with LGE imaging.
Why the study?
Does a segmented MLEV phase-cycled T2 preparation sequence provide reproducible T2 mapping of healthy and diseased mouse myocardium?
Does a segmented MLEV phase-cycled T2 preparation sequence provide reproducible T2 mapping of healthy and diseased mouse myocardium?
A segmented MLEV phase-cycled T2 preparation sequence provides high-quality, reproducible T2 maps for assessing myocardial edema and infarct size in mouse models.
Supports T2 mapping in mouse infarct models; leaves open translation to clinical cardiac MRI.
PURPOSE: A high-quality, reproducible, multi-slice T2-mapping protocol for the mouse heart is presented. METHODS: A T2-prepared sequence with composite 90° and 180° radiofrequency pulses in a segmented MLEV phase cycling scheme was developed. The T2-mapping protocol was optimized using simulations and evaluated with phantoms. RESULTS: Repeatability for determination of myocardial T2 values was assessed in vivo in n = 5 healthy mice on 2 different days. The average baseline T2 of the left ventricular myocardium was 22.5 ± 1.7 ms. The repeatability coefficient for R2 = 1/T2 for measurements at different days was ΔR2 = 6.3 s(−1). Subsequently, T2 mapping was applied in comparison to late-gadolinium-enhancement (LGE) imaging, to assess 1-day-old ischemia/reperfusion (IR) myocardial injury in n = 8 mice. T2 in the infarcts was significantly higher than in remote tissue, whereas remote tissue was not significantly different from baseline. Infarct sizes based on T2 versus LGE showed strong correlation. To assess the time-course of T2 changes in the infarcts, T2 mapping was performed at day 1, 3, and 7 after IR injury in a separate group of mice (n = 16). T2 was highest at day 3, in agreement with the expected time course of edema formation and resolution after myocardial infarction. CONCLUSION: T2 prepared imaging provides high quality reproducible T2 maps of healthy and diseased mouse myocardium.
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Coolen et al. (2013) studied Ischemia/reperfusion myocardial injury (n=29). Segmented MLEV phase-cycled T2 preparation vs. Late-gadolinium-enhancement (LGE) imaging was evaluated on Myocardial T2 values and infarct size correlation with LGE. T2-prepared imaging provided reproducible T2 maps of mouse myocardium, with infarct T2 significantly higher than remote tissue and infarct sizes strongly correlating with LGE imaging.