An investigation of the post‐tetanic potentiation of end‐plate potentials at a mammalian neuromuscular junction

Curarized or Mg-paralysed rat diaphragm-phrenic nerve preparations in vitro

Tetanic stimulation (1000 impulses at 100/sec) under various experimental conditions (polarizing currents, altered NaCl, ethyl alcohol, pH changes, metabolic inhibitors, cardiac glycosides, altered glucose/potassium, osmotic changes)

Control conditions (pre-tetanus or standard bathing solution)

Amplitude of end-plate potentials (e.p.p.s) and post-tetanic potentiation (P.T.P.)surrogate